Journal: Cell Reports

Article Title: Trained immunity induction by the inactivated mucosal vaccine MV130 protects against experimental viral respiratory infections

doi: 10.1016/j.celrep.2021.110184

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Article Snippet: BV786 Rat anti-mouse CD45R/B220 , BD Biosciences , Cat# 563894;RRID: AB_2738472.

Techniques: Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Lactate Assay, Purification, Sequencing, Expressing, Software

Journal: Immunity

Article Title: Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication

doi: 10.1016/j.immuni.2022.05.013

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Article Snippet: BV786 rat anti-mouse B220 (clone RA3-6B2) , BD Bioscience , Cat#: 563894; RRID: AB_2738472.

Techniques: Produced, Subcloning, Recombinant, Electron Microscopy, Protease Inhibitor, Purification, Modification, Selection, Western Blot, In Situ, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Bradford Protein Assay, Mouse Assay, Sequencing, Real-time Polymerase Chain Reaction, Clone Assay, Software

Journal:

Article Title: Interleukin 18 together with interleukin 12 inhibits IgE production by induction of interferon-? production from activated B cells

doi:

Figure Lengend Snippet: Induction of spleen cells, B cells, and non-B cells to develop into cells expressing IFN-γ mRNA by injection of IL-12 and IL-18. Highly purified B cells (108) from IFN-γ+/+ C57BL/6 mice were transferred into IFN-γ−/− mice. IL-12 (100 ng/mouse) and IL-18 (1,000 ng/mouse) were injected i.p. every day for 4 days into IFN-γ+/+ mice (Nos. 1–3), IFN-γ+/− mice or IFN-γ−/− mice (Nos. 1, 2) administered with IFN-γ+/+ B cells. mRNAs extracted from whole spleen cells (W), B220+ cells (B), and B220− cells (ΔB) fractionated from whole spleen cells by using magnetic beads coated with rat anti-mouse B220 antibody were amplified by RT–PCR for analysis of expression of IFN-γ mRNA. Flow cytometric analysis revealed that more than 95% of beads bound cells (B) and less than 0.5% of beads-unbound cells (ΔB) were surface IgM positive.

Article Snippet: Magnetic beads coated with rat anti-mouse B220 antibody were purchased from PerSeptive Diagnostics (Cambridge, MA).

Techniques: Expressing, Injection, Purification, Magnetic Beads, Amplification, Reverse Transcription Polymerase Chain Reaction